Figure 6.

Mitochondrial respiratory chain protein expression. (A) Representative blots and densitometry of selected protein data (complex I and complex IV) from soleus muscle at both disease stages. (B) Representative blots and densitometry of selected protein data (ND1 and COX I) from soleus muscle at both disease stages. Homogenates of skeletal muscle were probed with antibodies detecting mitochondrial respiratory chain complexes I–V (total OXPHOS), the complex I proteins ND1 and NDUFS1, and the complex IV proteins COX I and COX IV. GAPDH served as a loading control. n = 6 samples per group. ^** P < 0.01; ^*** P < 0.001. AOB, ascending aortic banding; PAB, pulmonary artery banding.