Table 1.
Primer sequences
| GenBank accession # | Forward primer | Reverse primer | |
|---|---|---|---|
| MuRF1 | NM_080903.1 | CTCCTTGTGCAAGGTGTTCG | AGTCTGAACTCGGTCGTTCC |
| Atrogin‐1 | NM_133521.1 | AGCTTGTGCGATGTTACCCA | GGTGAAAGTGAGACGGAGCA |
| Myostatin | NM_019151.1 | TATCACGCTACCACGGAAACAA | ATCCACAGCTGGGCCTTTAC |
| PGC‐1α | NM_031347.1 | CCGAGAATTCATGGAGCAAT | GTGTGAGGAGGGTCATCGTT |
| IGF‐1 | NM_001082477.2 | GGACGCTCTTCAGTTCGTGT | GATCACAGCTCCGGAAGCAA |
| IL‐1β | NM_031512.2 | CTATGTCTTGCCCGTGGAGC | CGTCATCATCCCACGAGTCA |
| IL‐6 | NM_012589.2 | CACAAGTCCGGAGAGGAGAC | TCTGACAGTGCATCATCGCT |
| TNF‐α | NM_012675.3 | TGATCCGAGATGTGGAACTGG | CGATCACCCCGAAGTTCAGTA |
| AT1 | NM_030985.4 | CCCACTCAAGCCTGTCTACG | CTGGCTTCTGTCAGGGCATTA |
| AT2 | NM_012494.3 | TTTGCCATCCTCCTGGGATT | GCCTTGGAGCCAAGTAATGG |
| 18S rRNA | NR_046237 | GTTGGTGGAGCGATTTGTCTG | GCTCAATCTCGGTGGCTGA |
AT1, angiotensin II receptor type 1; AT2, angiotensin II receptor type 2; IGF‐1, insulin‐like growth factor 1; IL, interleukin; MuRF1, muscle‐specific RING finger 1; PGC‐1α, peroxisome proliferator‐activated receptor‐γ coactivator 1α; TNF‐α, tumour necrosis factor‐α.