Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan;27(1):66–79. doi: 10.1261/rna.076810.120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Mukhopadhyay et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

FIGURE 5. — Catalytically inactive PUS10 selectively binds only certain other tRNAs. (A) Radiolabeled tRNAs for Trp, Gln, Ala, and Phe (indicated below the panels) were incubated with increasing concentrations of h-PUS10, resolved by native PAGE, and visualized by phosphorimaging. (B) Reactions similar to those in A using i-PUS10 with tRNA^Trp and tRNA^Ala. (C) Reactions similar to those in A using h-PUS10 with tRNA^iMet and tRNA^AU-Ala and their mutants (mut-iMet and mut-AU-Ala) where sequences at positions 59–60 were interchanged as described in the text. (D) Separately radiolabeled tRNA^Trp (*Trp) along with increasing concentrations of unlabeled tRNAs (indicated above the panels) for Trp, Gln, initiator Met, and Phe were incubated with h-PUS (20 µM), resolved by native PAGE, and visualized by phosphorimaging. The first lane in each panel contains only the radiolabeled tRNA^Trp, with no unlabeled tRNA or h-PUS10. (E) Reactions similar to A after separate incubations of tRNA^Trp with nh-PUS10 and ch-PUS10.