FIGURE 4.

In vitro translation of a full-length protein by domain IV mutant EF-Gs. A mRNA coding for ribosomal protein S2 was translated in vitro by 70S ribosomes in an E. coli system (Ali et al. 2006) using mutant forms of EF-G with S100 extract (A) containing or (B) lacking endogenous wild-type EF-G. (WT) Addition of wild-type EF-G; (No EF-G) wild-type EF-G not added; (S2) position of full-length protein S2; (23 kD) a translation product likely made from a 3'-truncated mRNA. All mutant EF-Gs except S587P and S588P are capable of catalyzing synthesis of full-length protein.