FIGURE 6.

Stimulation of −1 frameshifting by domain IV mutants. SDS gels showing in vitro translation of [³⁵S]-labeled ribosomal protein S2 from a mRNA containing a “slippery sequence” with domain IV mutants using S100 extract (A) containing wild-type EF-G and (C) lacking wild-type EF-G. (B,D) Histograms showing frameshifting efficiencies for A and C, respectively, plotted as the ratio of frameshifted product to 0-frame product. (S2) Full-length S2; (23 kDa) carboxy-terminal truncated S2 product; (FS) −1 frameshift product; (WT) wild-type EF-G. Error bars represent standard errors of the mean.