Figure 2.

CRISPR/Cas9-mediated generation of Rabl2 mutant mice. (A) Diagram of CRISPR-Cas9 genome editing strategy to introduce the p.Leu119Phe and p.Val158Phe amino acid changes into mouse Rabl2. The human RABL2A p.Leu119Phe (rs80006029) and p.Val158Phe (rs200121688) variants are located in exons 5 and 6, respectively. (B) Representative Sanger sequencing chromatograms from WT, Rabl2^L119F/L119F, Rabl2^V158F/V158F and Rabl2^−/− mice. The relevant codon bases are labeled above. Black arrow above the chromatogram indicates the insertion. (C) qRT-PCR analysis of Rabl2 expression in testis. Data in C are represented as the mean ± SEM and were analyzed using one-way analysis of variance (ANOVA) with Tukey’s post hoc test.