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. 2020 Apr 1;12(9):688–699. doi: 10.1093/jmcb/mjaa011

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© The Author(s) (2020). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Pten inactivation due to Asxl1 loss contributes to IL3-independed growth of 32D cells. (A) RT-qPCR analysis of relative mRNA levels of Pten after IL3 withdrawal at the specified time points. (B and C) Expression levels of Pten in the presence or absence of IL3 for 24 h were determined by RT-qPCR (B) and WB (C) analyses. (D) WB analysis of ectopic expression of Flag-tagged PTEN and PTEN C124S as well as p-AKT 473 and total AKT. (E) Cell growth curves of designated groups of 32D cells after withdrawal of IL3 at the specified time points. Significance level was determined using Student’s two-sided t-tests. The error bars denote SD, n = 3; **P ≤ 0.01, NS indicates not significant.