FIGURE 3.

The infectivity of chimeric T36CA‐T36FL virus (Hyb1). Chimeric vector pHyb1 (Figure 2) and pT36FL were inoculated to transgenic (HC‐Pro) Nicotiana benthamiana plants by agroinfiltration and evaluated for bioactivity. (a) Green fluorescent protein (GFP) expression was observed in both the infiltrated and systemic leaves, at 9 days postinoculation (dpi) and 6 weeks postinoculation (wpi), respectively, under UV illumination with the leaves attached to the plants. (b) Representative images showing systemic symptoms characterized by leaf epinasty (left) and vein chlorosis in Hyb1‐infected N. benthamiana plants at 10 wpi. (c) Virion accumulation in the systemic leaves of infected N. benthamiana plants over time was determined by double antibody sandwich‐ELISA. The average absorbance at 405 nm (A₄₀₅) is calculated from a total of six individual samples from two repeated experiments and error bars represent the SE. (d) A transmission electron micrograph (with scale bar) showing virion‐like particles of Hyb1 purified from infected N. benthamiana. (e) A representative wide‐field fluorescence microscopy image of GFP signals observed in the peeled bark of a newly developed branch of a Citrus macrophylla plant inoculated with Hyb1 virion preparation at 10 wpi