FIGURE 5.

Reactive oxygen species (ROS) and nitric oxide (NO) levels in stomata ofAtran1mutant plants after chitin treatment. (a) H₂DCF‐DA was loaded into the epidermal peels cells, and fluorescence images and pixel intensities were recorded in the guard cells of Col‐0,Atran1mutant, and g AtRAN1plants after addition of 50 µg/ml chitin. (b) DAF‐2DA was loaded into epidermal peels cells, and fluorescence images and pixel intensities were recorded in guard cells of Col‐0,Atran1mutant, and gAtRAN1plants after addition of 50 µg/ml chitin. Each measurement is the average of three replicates; experiments were repeated twice with similar results. Data for fluorescence intensity of ROS and NO generation are presented as means ± SEof three independent experiments