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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Brain Behav Immun. 2020 Sep 19;91:587–600. doi: 10.1016/j.bbi.2020.09.016

Figure 3. Intranasal administration of INT-777 reduced microglial NLRP3-ASC inflammasome activation-mediated neuroinflammation within ipsilateral hemisphere at 24 h after SAH.

Figure 3.

(A) Representative immunofluorescence microphotographs of microglia (Iba-1, green) and CD68 (red)-positive activated microglia in the ipsilateral basal cortex for sham, SAH+Vehicle, and SAH+INT-777 groups. Nuclei were stained with DAPI (blue). Scale bar=100 μm (J) A small black squares in the coronal section of brain indicated the area used for microphotographs. (C-D) Quantitative analysis of Iba-1-positive and CD68-positive microglia. n=4 per group. (B, E-I) Representative western blot bands and densitometric quantification of NLRP3, ASC, IL-1β, IL-6, and TNF-a in the ipsilateral hemisphere at 24 h after SAH, n=6 per group. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group; @P<0.05 vs. SAH+Vehicle group.