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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Brain Behav Immun. 2020 Nov 4;91:437–450. doi: 10.1016/j.bbi.2020.11.001

Fig. 1.

Fig. 1.

Study Design. C57BL/6J mice were orally gavaged with antibiotic (Abx) cocktail or water (vehicle) from postnatal (P) day 7 to P23. From P24 to P50, mice were given normal water or tributyrin. Upon euthanasia, fecal pellets were used to analyze gut bacterial community analysis via 16s Illumina sequencing. Tissue samples such as prefrontal cortex (PFC), hippocampus, and ileum were used for qPCR or western blot. Immunofluorescence was performed in the medial-PFC. Ileal physiology was analyzed via Ussing chambers. Behavioral tests were performed to assess cognition and anxiety-like behavior. Neurogenesis was analyzed via immunofluorescence of dorsal hippocampus.