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. Author manuscript; available in PMC: 2021 Dec 20.
Published before final editing as: Stem Cell Res. 2020 Jun 20;47:101869. doi: 10.1016/j.scr.2020.101869

Figure 3. HSC from WT and Gpr68−/− mice exhibit comparable competitiveness.

Figure 3.

(A)Schematics of experimental approach for primary (1°) and secondary (2°) cBMT. (B) Representative flow cytometric analysis of donor-derived (CD45.2+) and competitor-derived (CD45.1+) hematopoietic cells in PB from recipients receiving BM cells from WT or Gpr68−/− mice (6 weeks old). (C) Frequency of CD45.2+ cells in PB from primary (1°) and secondary (2°) recipients receiving BM cells from WT or Gpr68−/− mice (6 weeks old, n=4~5). (D-F) Frequency of CD45.2+ cells in BM HSPC subpopulations from primary recipients receiving BM cells from WT or Gpr68−/− mice (6 weeks old, n=4). (G) Frequency of CD45.2+ cells in PB from primary (1°) recipients receiving BM cells from WT or Gpr68−/− mice at older age (10~11 months old, n=6).