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. Author manuscript; available in PMC: 2021 Dec 20.
Published before final editing as: Stem Cell Res. 2020 Jun 20;47:101869. doi: 10.1016/j.scr.2020.101869

Figure 4. Signaling pathways in HSPC from Gpr68−/− mice.

Figure 4.

(A)Representative flow cytometric analysis of cytosolic Ca2+ levels in slam+ cells from WT and Gpr68−/− mice (4~5 months old). (B) Cytosolic Ca2+ levels in slam+ cells from WT and Gpr68−/− mice at young age (4~5 months old, n=5, left panel) and upon aging (10~11 months old, n=5, right panel). (C) Cytosolic cAMP levels in lineage- cells from WT and Gpr68−/− mice (4~5 months old, n=2). (D) Representative flow cytometric analysis of Gpr68 expression on hematopoietic cells (CD45+), non-hematopoietic cells (Non-hem) and CD31+ cells in BM from C57Bl6 mice (5-month old). (E) Gpr68 protein expression on hematopoietic cells (CD45+), non-hematopoietic cells (Non-hem), CD31+ and CD31- cells in BM from C57Bl6 mice (5-month old, n=4). (F) Relative mRNA levels of Gpr4, Gpr65 and Gpr132 in LSK cells from WT and Gpr68−/− mice (7-month old, n=3).