Figure 5. Characterization of wCfeJ and wCfeJ in cat fleas.

(A) Screening for wolbachiae in geographically diverse cat flea colonies (top) and wild populations (bottom). Cat fleas from six locations (including two strains from Sierra Research Laboratories) were assessed for the presence of wCfeT and wCfeJ. Wolbachia infection of individual fleas was assessed by qPCR (see “Materials and Methods” for details). (B) Localization of wCfeT and wCfeJ in cat flea tissues. Dissected ovaries and other tissues (mostly midguts) from individual cat fleas (EL Colony) were assayed by qPCR (see “Materials and Methods” for details). Five fleas were assayed for each tissue type.