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. 2020 Dec 7;10:607253. doi: 10.3389/fcimb.2020.607253

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Copyright © 2020 Telittchenko and Descoteaux

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Survival of mating crosses within infected macrophages and visualization of both parental strains within the same vacuole. (A) BMMs were infected with metacyclic serum-opsonized promastigote crosses of L. mexicana complex parental parasite strains (L. amazonensis LPG2/LPG2::ΔHYG + L. amazonensis +/SSU::NEO-GFP; L. amazonensis LPG2/LPG2::ΔHYG + L. amazonensis NEO-DsRede; L. mexicana LPG2/LPG2::ΔHYG + L. amazonensis +/SSU::NEO-GFP for 2, 48, 120, and 196 h. Bars represent mean ± SE of three representative experiments performed in triplicate in bone marrow derived murine macrophages. Parasites were counted in 100 macrophages and quantified by light microscopy. Macrophages were stained with HEMA 3 kit. Representative pictures from each cross are shown. (B) Live microscopy analysis of L. amazonensis and L. mexicana parasite strains expressing different fluorescent markers. Representative pictures of both parental strains within the same communal vacuole at 48 and 72 h are shown. LV79-GFP, L. amazonensis HYG-GFPe; LV79-DsRed, L. amazonensis NEO-DsRede; M379-DsRed, L. mexicana NEO-DsRede.