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. 2020 Dec 7;14:584928. doi: 10.3389/fncel.2020.584928

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Copyright © 2020 Li, Liu, Zhao and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of recording site using fluorescent phalloidin. (A) Superimposed confocal and DIC images (10 × lens) obtained from a P6 utricle. Fluorescent phalloidin was injected to the recording site by applying a positive pressure to the recording electrode after ELP was recorded. The area with fluorescent signal is marked by a red arrow. A white arrow marks otoconia in a nearby area. Scale bar: 100 μm [for (A), (B) and (C) panels]. (B) Superimposed confocal and DIC images of a P6 saccule. A red arrow marks the area with fluorescent signal (recording site) while a white arrow marks otoconia. (C) Superimposed confocal and DIC images from a P30 saccule. The area with a frame is presented in panel D with higher magnification. (D) Superimposed confocal and DIC images (63 × lens) of phalloidin-labeled stereocilia bundles of saccular hair cells. Otoconia can also been seen. Scala bar: 10 μm.