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. 2020 Dec 7;8:603259. doi: 10.3389/fchem.2020.603259

Figure 4.

Figure 4

TIRF-based single-molecule tracking to investigate membrane transient interactions. (A) Protein homodimerization and lipid–lipid interactions are transient process that can be studied with TIRF. (B) Transient interactions between proapoptotic BH3 protein and membrane-bound antiapoptotic Bcl-2 proteins can be studied using FLIM-FRET. FRET among Venus and mCherry facilitates Venus relaxation and lowers its lifetime.