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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Pharm Sci. 2020 Sep 12;110(1):412–421. doi: 10.1016/j.xphs.2020.09.013

Figure 1. Effect of pregnancy-related hormones on mRNA levels of key CYP isoforms in SCHH.

Figure 1.

Human hepatocytes from three female donors (HU1880, HC3–26 and HC5–40) were exposed to hormones (E2, E3, E4, P4, CRT), either individually or in combination as a cocktail [CKTL] of all hormones, or controls (DMSO, CITCO, Rifampin [RIF]) for 72 h (n=2/group within each donor). (A) CYP3A4, (B) CYP2B6 and (C) CYP2C8 mRNA levels were quantified, normalized to GAPDH, expressed relative to vehicle (DMSO) control within each donor, and then combined for comparison across experimental groups (n=3 donors/group; mean ± SEM; *p<0.05 vs. DMSO). Concentration-dependent effects were evaluated (open bar: 1 μM, solid bar: 10 μM; ^p<0.05 1 vs. 10 μM). (D-F) Correlation between CYP mRNA levels and protein concentrations quantified by QTAP in SCHH membrane-associated protein in donors HU1880 and HC3–26. Data points represent the mean fold-changes in expression for each treatment group, relative to DMSO, within each donor. The Pearson correlation coefficient (r) and corresponding p-values are provided.