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. 2020 Dec 1;38:101822. doi: 10.1016/j.redox.2020.101822

Fig. 1.

Fig. 1

Oxidation of aLA (100 μM) by different sources of 1O2 (panel A: 10 μM Rose Bengal; panel B: 50 μM methylene blue; panel C: 35 μM riboflavin; in the presence of visible light and O2 in each case) occurs in a time-dependent manner, and the intermediates react with GSH (10 mM) to give modified aLA species. Panel A: Representative SDS-PAGE of aLA after photo-oxidation by Rose Bengal and then reaction with GSH; Panel B: Representative SDS-PAGE of aLA after photo-oxidation by methylene blue and then reaction with GSH; Panel C: Representative SDS-PAGE of aLA after photo-oxidation by riboflavin and then reaction with GSH. Images are representative of three independent experiments.