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. 2020 Dec 16;14(2):100983. doi: 10.1016/j.tranon.2020.100983

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© 2020 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 1 — High-dose radiation (three 12-Gy fractions) led to increased numbers of plasmacytoid dendritic cells (pDCs) at tumor sites in a mouse model of lung cancer. (A) Mice were inoculated in the right hind legs with 344SQ non-small cell lung adenocarcinoma cells to establish tumors and then deliver high-dose radiotherapy (RT) as shown. Tumors were harvested, processed, and analyzed by flow cytometry at 3 days (n = 5 samples) and 5 days (n = 5 samples) after the final fraction of RT (arrows). (B, C) Percentages and absolute cell numbers of pDCs were analyzed by gating on CD45⁺ population, followed by CD11c⁺ PDCA-1⁺ subpopulation, as also shown by the dot plots. (D) Percentages and absolute numbers of DCs were analyzed using CD11c⁺ CD103⁺ markers. (E, F) Percentages and absolute numbers of CD4⁺ T cells and CD49b⁺ natural killer (NK) cells were analyzed. Student t-tests were conducted to compare statistical significance between groups. *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.