Fig. 2.

Radiation followed by CMP-001 led to inhibited tumor growth, improved survival rates, and reduced lung metastases in a mouse model of lung cancer. (A) Mice were inoculated in the hind legs with 344SQ cancer cells, with the right leg considered the “primary” tumor (and therefore irradiated with high-dose radiotherapy [RT]) and the left leg considered the abscopal tumor (and therefore left unirradiated). Mice were treated with RT, CMP-001, or RT+ CMP-001 as shown (B) Kaplan-Meier survival curves indicated prolonged survival of mice treated with high-dose radiotherapy (RT; three 12-Gy fractions on days 6, 7, and 8) followed by three IT doses of CMP-001 given on days 11, 15, and 20. (C and D) Growth curves for “primary” (right leg, irradiated) tumors (C) and “secondary” (abscopal, unirradiated) tumors (D), showed that high-dose RT followed by adjuvant CMP-001 had the greatest antitumor effect of the indicated treatments. (E) High-dose RT followed by adjuvant CMP-001 led to decreased numbers of lung metastases. Data are expressed as mean diameters from each mouse at each time point and reported as Mean ± SEM. Experiments were conducted twice, and data was pooled for survival and tumor growth.