Fig. 2.

Effects of Erlotinib, Hex and their combination on EGFR phosphorylation (Tyr1068).

Levels of p

(Tyr1068)-EGFR were measured by Western blot. Caco-2 cells were added with 100 and 500 nM Erlotinib, 2.5 and 5 μM Hex, and their simultaneous combination. After 30 min incubation at 37 °C, cells were incubated for further 10 min in the absence or the presence of EGF (10 ng/ml). After Western blots, bands were quantified and values for p (Tyr1068)-EGFR referred to total EGFR content. Results were referred to control values (1, dashed line). Results are shown as mean ± SEM and are the average of at least five independent experiments. Values having different superscripts are significantly different (p < 0.05, One way ANOVA).