Fig. 6.

Effects of Hex on EGFR distribution and Tyr 1068 phosphorylation in lipid rafts.

Detergent-free lipid rafts were isolated from Caco-2 cells treated with/without Hex (5, 10 μM) for 30 min at 37 °C and incubated in the absence/presence of EGF (10 ng/ml) for 10 min at 37 °C. Gradients were separated into 6 fractions and each fraction was analyzed by SDS-PAGE and immunoblotted with the indicated antibodies. (A) Representative images for EGFR localization and phosphorylation at lipid rafts. After quantification of bands in fraction 2 (lipid rafts fraction) EGFR (B) and p-(Tyr1068)-EGFR (C) levels were referred to flotillin-1 and total EGFR content, respectively. (B) Results were referred to control values (1, dashed line). Results are shown as means ± SEM of four independent experiments. Values having different superscripts are significantly different (p < 0.05, One way ANOVA).