Table 1.

List of SP sequences with essential amino acids (EAAs, various colors) and non-essential amino acids (NEAAs, uncoloured) usage shown.

Family	Signal peptide amino acid position																						Total variety/No. of EAA
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22
Vκ1	M	D	M	R	V	P	A	Q	L	L	G	L	L	L	L	W	L	P	G	A	R	C	4/11
Vκ2	M	R	L	P	A	Q	L	L	G	L	L	M	L	W	V	P	G	S	S	A			4/10
Vκ3	M	E	A	P	A	Q	L	L	F	L	L	L	L	W	L	P	D	T	T	G			5/12
Vκ4	M	V	L	Q	T	Q	V	F	I	S	L	L	L	W	I	S	G	A	Y	G			7/12
Vκ5	M	G	S	Q	V	H	L	L	S	F	L	L	L	W	I	S	D	T	R	A			8/12
Vκ6	M	L	P	S	Q	L	I	G	F	L	L	L	W	V	P	A	S	R	G				6/10
VH1	M	D	W	T	W	R	I	L	F	L	V	A	A	A	T	G	A	H	S				8/11
VH2	M	D	I	L	C	S	T	L	L	L	L	T	V	P	S	W	V	L	S				6/13
VH3	M	E	F	G	L	S	W	V	F	L	V	A	I	L	K	G	V	Q	C				7/12
VH4	M	K	H	L	W	F	F	L	L	L	V	A	A	P	R	W	V	L	S				7/14
VH5	M	G	S	T	A	I	L	A	L	L	L	A	V	L	Q	G	V	C	S				5/10
VH6	M	S	V	S	F	L	I	F	L	P	V	L	G	L	P	W	G	V	L	S			6/13
VH7	M	D	W	T	W	R	I	L	F	L	V	A	A	A	T	G	A	H	S				8/11
IgE	M	D	W	T	W	I	L	F	L	V	A	A	A	T	R	V	H	S					8/12

Comparisons of the total variety counts of EAAs in the SPs explored in this study.