Table 2.
Amino acid counts in DMEM media and the representative average of amino acid usage in all the antibodies in this study.
| AA counts in full-length antibody | Number of AA in DMEM media (1020) | Maximum number of possible antibodies produced (1018) | ||
|---|---|---|---|---|
| EAAs | Phenylalanine (F) | 50 | 2.41 | 4.82 |
| Histidine (H) | 26 | 1.63 | 6.27 | |
| Isoleucine (I) | 34 | 4.82 | 14.18 | |
| Lysine (K) | 92 | 6.01 | 6.53 | |
| Leucine (L) | 116 | 4.82 | 4.16 | |
| Methionine (M) | 14 | 1.21 | 8.64 | |
| Threonine (T) | 112 | 4.80 | 4.29 | |
| Valine (V) | 128 | 4.83 | 3.77 | |
| Tryptophan (W) | 25 | 0.47 | 1.88 | |
| NEAAs | Alanine (A) | 68 | 0 | 0 |
| Tyrosine (Y) | 62 | 3.45 | 5.56 | |
| Serine (S) | 160 | 2.41 | 1.51 | |
| Cysteine (C) | 32 | 3.11 | 9.72 | |
| Glycine (G) | 84 | 2.41 | 2.87 | |
| Aspartic Acid (D) | 58 | 0 | 0 | |
| Asparagine (N) | 51 | 0 | 0 | |
| Glutamic Acid (E) | 62 | 0 | 0 | |
| Proline (P) | 96 | 0 | 0 | |
| Glutamine(Q) | 63 | 24.07 | 38.21 | |
| Arginine (R) | 35 | 2.91 | 8.31 |
The number of amino acids in DMEM media were calculated based on the weight (g/L) of amino acid component used in the formula. Maximum number of antibodies produced refers to the theoretical maximum number of full-length antibodies that could be synthesized based on number of amino acids in DMEM media and to the amino acid counts in the representative average of amino acids.