FIGURE 1.

Library construction and 3D pooling strategies. (A) Library construction strategy. Superscript letters correspond to citations: a: (Liles et al., 2008); b: (Kakirde et al., 2011); c: (Nasrin et al., 2018). (B) 3D Library Pooling strategy: Plate, Column, and Row are barcoded before sequencing. (C) Contig to clone deconvolution and in silico mining strategy. Reads from each pool are assembled separately into pool contigs. Highly homologous contigs from each dimension (Plate, Column, and Row) form triplets. Since their pool of origin is known, they form a coordinate system that points to their clone of origin. Contigs were analyzed bioinformatically for rational identification of candidates for functional screening.