Skip to main content
. 2020 Dec 7;11:594390. doi: 10.3389/fimmu.2020.594390

Figure 6.

Figure 6

Analysis of the apoptosis dynamics of granulocytes and hyalinocytes post WSSV infection by flow cytometry and IFA. (A) IFA analysis of apoptotic hemocyte subpopulations sampled at 48 hpi. A1: DAPI stained the nucleus of hemocytes; A2: TUNEL stained apoptotic hemocytes; A3: Alexa Fluor®488 stained granulocytes; A4: The merge of A1, A2 and A3. Bars=10 μm. The yellow arrows indicate apoptotic granulocytes; the green arrows indicate non-apoptotic granulocytes; the red arrows indicate apoptotic hyalinocytes. (B) B1: Hemocytes were gated (R1) on FSC/SSC dot plots. B2-B8: Representative dot plots showing apoptotic rates of different hemocyte subpopulations at 0, 12, 24, 36, 48, 60 and 72 hpi. Q2-LL: non-apoptotic hyalinocytes; Q2-LR: non-apoptotic granulocytes; Q2-UL: apoptotic hyalinocytes; Q2-UR: apoptotic granulocytes. (C) The apoptotic rates of granulocytes and hyalinocytes. All values are given as arithmetic mean ± S.D (n = 3). Significant differences between the two hemocyte subpopulations are marked with asterisks, p <0.05.