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. 2020 Dec 7;11:593468. doi: 10.3389/fphys.2020.593468

FIGURE 2.

FIGURE 2

The effects of 5FU on skeletal muscle immune cells. (A) Cells were gated for non-debris (SSC-A × FSC-A), (B) FSC singlets (FSC-W × FSC-H), (C) and SSC singlets (SSC-W × SSC-H; A-right). (D) Non-debris singlet cells were then gated for total immune cells with CD45+. (E) CD45+ cells were quantified and shown in the bar graph as the relative % of non-debris singlets. (F) CD45+ cells were gated for monocytes with CD11b+. (G) CD45+CD11b+ cells were quantified and shown in the bar graph as the relative % of CD45+ cells. (H) CD45+CD11b+ were then gated for macrophages with F4/80. (I) F4/80+ cells were quantified and shown in the bar graph as the relative % of CD45+CD11b+ cells. Significance was set at p < 0.05. *Significantly different from Control using a student’s t-test.