FIGURE 4.

The effects of 5FU on infiltrating skeletal muscle monocytes and macrophages. (A) CD11b+ monocytes were gated for their activation status using Ly6C. Cells were considered either resident (Ly6cLow) or activated/infiltrating (Ly6cHigh). (B) Ly6cHigh monocytes were quantified and shown in the bar graph as relative % of CD45+CD11b+ cells. (C) F4/80+ macrophages were gated analyzed for their polarization status using CD11c and CD206. (D) CD11c-CD206- cells were considered M0-like macrophages, CD11c+CD206- cells were considered M1-like macrophages, CD11c-CD206+ cells were considered M2-like macrophages, and CD11c+CD206+ cells were considered M1-M2-like transitional macrophages and graphed as the relative % of F480+ macrophages. Significance was set at p < 0.05.*Significantly different from control (t-test).