Skip to main content
. 2020 Dec 7;8:594528. doi: 10.3389/fcell.2020.594528

FIGURE 3.

FIGURE 3

RAP2a and DNMT3a are the direct targets of miR-199a-3p. (A) Schematic of the putative miR-199a-3p target site in the human RAP2a 3′-UTR. (B) Schematic of the putative miR-199a-3p target site in the human DNMT3a 3′-UTR. (C) KTC-1 cells were co-transfected with the luciferase reporter carrying the WT-RAP2a-3′-UTR or MUT- RAP2a -3′-UTR, and miR-199a-3p or control miR mimics. Firefly luciferase values obtained 48 h after transfection and normalized to Renilla luciferase are presented. (D) BCPAP cells were co-transfected with the luciferase reporter carrying WT-DNMT3a-3′-UTR or MUT- DNMT3a-3′-UTR, and miR-199a-3p or control miR mimics. Firefly luciferase values obtained 48 h after transfection and normalized to Renilla luciferase are presented. (E) RAP2a and DNMT3a protein levels obtained by Western blot analysis (30 μg of protein was used for each Western blot performed) were tested 2 days after BCPAP cells were transfected with miR-199a-3p mimics, mimic controls, miR-199a-3p inhibitors, or inhibitor controls. *p < 0.05.