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. 2020 Dec 7;8:594528. doi: 10.3389/fcell.2020.594528

FIGURE 4.

FIGURE 4

Rap2a promotes cancer cell migration, invasion, and growth. (A) The inhibitory efficiency of siRNAs targeting RAP2a was verified by western blotting. (B) Migration of BCPAP cells was tested by using the Transwell migration assay. (C) Quantitative analysis of the migrated cells in (B). n = 3 per group. (D) The invasiveness of BCPAP cells was tested by using the Transwell invasion assay. (E) Quantitative analysis of the invasive cells in (D). n = 3 per group. (F) BCPAP cells were transfected with either negative control or siRAP2a for 24 h, and 1,000 cells were seeded into six-well plates for 7 days to assay colony-formation ability. (G) The colonies were counted, and the results are presented as mean ± S.D. from three individual experiments. (H) The expression of RAP2a in non-cancerous thyroid tissues and PTC tissues was assayed by immunohistochemistry staining. (I) qRT-PCR showing the expression of miR-199a-3p in non-cancerous thyroid tissues and PTC tissues. n = 60. (J) Scatter plots of miR-199a-3p expression against the RAP2a level. *p < 0.05. Bars = 200 μm.