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. 2020 Dec 17;25:100876. doi: 10.1016/j.bbrep.2020.100876

Table 1.

Purification of cysteine proteinase inhibitor from E. contortisiliquum seeds.

Steps Volume (mL) Protein (mg/mL) Total protein (mg) aUI/mL bUI/total cEA dPurif. Yield (%)
Precipitation at 80% acetone 400 2.9 1160 50 20,000 17.24 1 100
DEAE-Sepharose 132 1.6 211.2 37.5 4950 23.44 1.36 24.75
Superdex 75 120 0.04 4.8 10 1.200 250 14.5 6
a

Inhibition Unit per mL.

b

Total Inhibition Unit (UI/mL x total volume).

c

Specific activity.

d

Fold purification.