Table 1.
Steps | Volume (mL) | Protein (mg/mL) | Total protein (mg) | aUI/mL | bUI/total | cEA | dPurif. | Yield (%) |
---|---|---|---|---|---|---|---|---|
Precipitation at 80% acetone | 400 | 2.9 | 1160 | 50 | 20,000 | 17.24 | 1 | 100 |
DEAE-Sepharose | 132 | 1.6 | 211.2 | 37.5 | 4950 | 23.44 | 1.36 | 24.75 |
Superdex 75 | 120 | 0.04 | 4.8 | 10 | 1.200 | 250 | 14.5 | 6 |
Inhibition Unit per mL.
Total Inhibition Unit (UI/mL x total volume).
Specific activity.
Fold purification.