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Biodistribution and pathological analysis. (A) Biodistribution of MF-MNB without FA modification, MF-MNB, and MF-MNB with FUS sonication for 3 min, determined by ICP-OES (n = 3), in major tissues of mice bearing 4T1 tumors after 4 and 24 h. (B) H&E-stained images of major organs from untreated healthy mice and treated mice with MF-MNB injection and FUS sonication taken 7 days and 21 days. No noticeable abnormality was observed in major organs (liver, spleen, kidney, heart, and lung).
