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Abundance and length distribution of small RNAs during intersegmental muscle development. Authentic microRNAs (miRNAs) that map to the genome are in black, while the gray bars reflect sequences that were not annotated. The majority of the small RNAs are ∼22 nucleotides long and map as miRNAs. The smaller peak at 27 nt may reflect piRNAs, a distinct class of small interfering RNAs. 20E, 20-hydroxyecdysone; D, day; PE, posteclosion.
