Fig. 2.
Comparison of the change from baseline (%) in mononuclear cell (MNC)-derived markers of oxidative stress and inflammation in women with PCOS before and after salsalate treatment in response to saturated fat ingestion (n = 8) [p47phox protein content (A), activated NF-κB (B), and IκBα protein content (C)] and in response to the hyperglycemic phase of the pancreatic clamp (n = 5) [p47phox protein content (D), activated NF-κB (E), and IκBα (F) protein content]. Representative Western blots in A, C, D, and F show the change in quantity of p47phox and IκBα in MNC homogenates, and the representative electrophoretic mobility shift assay bands in B and E show the change in quantity of NF-κB in nuclear extracts from MNC in samples collected at 0 and 2 h after the respective pro-oxidant or proinflammatory triggers. The samples used to quantify p47phox and IκBα protein content and activated NF-κB by densitometry were run on the same gel. Data are presented as means ± SE. *Pretreatment significantly different compared with posttreatment (paired Student’s t test); P < 0.0001 (A), P < 0.0007 (B), P < 0.0003 (C), P < 0.002 (D), and P < 0.04 (E) and (F).