Amplification of the STX17 gene using genomic DNA extracted from
hair roots stored for a long term. Genomic DNA was extracted with spin columns (A)
or silica beads (B) methods. F_2010_1, F_2010_2, and F_2010_3 were stored for 10
years at 4–8°C; F_2012_1 was stored for 8 years at 4–8°C; F_2015_1 and F_2015_2 were
stored for 5 years at 4–8°C; and F_2017_1 was stored for 3 years at 4–8°C. R_2009_1,
R_2009_2. R_2009_3 was stored for 11 years at room temperature; R_2010_1, R_2010_2,
and R_2010_3 were stored for 10 years at room temperature; R_2011_1, R_2011_2, and
R_2011_3 were stored for 9 years at room temperature; and R_2016_1, R_2016_2, and
R_2016_3 were stored for 4 years at room temperature.