Fig. 1.

Localization of satellite DNA and transposable elements on metaphase chromosomes of R. acetosa using FISH. Scale bars = 10 µm. (A) RAE180 (red signal) and RAE173 satellite (green signal) paint almost the entire Y chromosomes. (B) RAYSIV satellite (red signal) is present on both Y chromosomes in two (Y₁) and three (Y₂) loci. (C) RAYSV satellite (red signal) is at one locus on the Y₁ chromosome in the subcentromeric region. (D) RAYSVI satellite (red signal) gives a signal at several discrete loci on both Y chromosomes. (E) RAYSVII satellite (red signal) is present in the distal part of the Y₁ chromosome. (F) RAE244 satellite (red signal) is accumulated on Y chromosomes and a few dispersed signals are observed in the remainder of the genome. (G) RAE93 (red signal) covers both Y chromosomes and a few loci in the remainder of the genome. (H) RA Ogre/TAT retrotransposon (red signal) is accumulated mostly on both Y chromosomes. (I) RA AleII retrotransposon is located on the distal parts of the Y₁ and X chromosomes. RAYSI satellite (green signal) was used as a Y-chromosome marker; the signal is localized in four spots on each arm of the Y₁ chromosome and in two spots on each arm of the Y₂ chromosome.