Table 2.

Effects of sodium-coupled neutral amino acid transporter 2 (SNAT2) suppression on the free amino acid profile of the C2C12 myotubes (μmol/L).

Item	Control	MeAIB	SEM	P-value
Essential amino acids
l-isoleucine	11.31 ± 0.61a	7.78 ± 0.45b	0.47	<0.01
l-leucine	11.92 ± 0.53a	8.34 ± 0.35b	0.39	<0.01
l-lysine	13.39 ± 0.15a	7.21 ± 0.46b	0.29	<0.01
l-methionine	3.25 ± 0.20a	2.17 ± 0.03b	0.12	<0.01
l-phenylalanine	7.96 ± 0.12a	5.16 ± 0.26b	0.18	<0.01
l-threonine	43.25 ± 1.04a	14.38 ± 0.64b	0.75	<0.01
l-tryptophan	2.29 ± 0.05a	1.43 ± 0.04b	0.04	<0.01
l-valine	15.41 ± 0.62a	10.00 ± 0.45b	0.47	<0.01
Non-essential amino acids
Cysteine	0.50 ± 0.05	0.29 ± 0.15	0.10	0.26
Glycine	288.10 ± 10.65a	178.30 ± 4.07b	6.98	<0.01
l-alanine	29.55 ± 0.90a	18.48 ± 0.32b	0.58	<0.01
l-arginine	7.35 ± 0.28a	4.63 ± 0.10b	0.18	<0.01
l-asparagine	3.60 ± 0.17a	1.31 ± 0.04b	0.11	<0.01
l-aspartic acid	7.23 ± 0.28a	2.49 ± 0.08b	0.18	<0.01
l-glutamic acid	139.19 ± 12.69a	96.63 ± 2.26b	7.90	0.03
l-glutamine	103.07 ± 1.47a	17.50 ± 0.26b	0.92	<0.01
l-histidine	3.92 ± 0.40a	2.56 ± 0.12b	0.26	0.03
l-proline	62.83 ± 2.15a	10.45 ± 0.61b	1.37	<0.01
l-serine	10.20 ± 0.31	8.35 ± 0.65	0.44	0.06
l-tyrosine	8.62 ± 0.41a	5.18 ± 0.11b	0.26	<0.01
Other amino acids
1-methyl-l-histidine	0.91 ± 0.02a	0.42 ± 0.02b	0.02	<0.01
Ethanolamine	10.52 ± 0.07	12.02 ± 0.58	0.36	0.06
Hydroxy-l-proline	1.28 ± 0.10a	0.67 ± 0.04b	0.07	<0.01
l-carnosine	5.11 ± 0.05a	1.43 ± 0.04b	0.09	<0.01
l-ornithine	1.02 ± 0.04a	0.57 ± 0.05b	0.04	<0.01
Taurine	204.17 ± 3.13a	182.39 ± 3.36b	2.81	<0.01
β-alanine	33.50 ± 0.57a	25.69 ± 0.36b	0.41	<0.01
γ-amino-n-butyric acid	0.75 ± 0.01a	0.44 ± 0.02b	0.02	<0.01

MeAIB = α-methylaminoisobutyric acid.

^{a, b} Means within the same row with different superscripts are significantly different (P < 0.05).