Fig. 2.

Growth restoration of the rnf mutant on H₂ + CO₂ after complementation with the plasmid pMTL_8312_Ptet_SrnfH. The A. woodii wild type (■), the wild type containing the vector pMTL8312_Ptet without the rnf operon (♦), the rnf mutant (▲), and the complemented strain Δrnf pMTL_8312_Ptet_SrnfH (▼) were grown in complex medium under a H₂ + CO₂ [80:20 v/v] atmosphere with a pressure of 1.0 × 10⁵ Pa. Plasmid-containing strains were induced with 400 ng/ml atet at an OD₆₀₀ of 0.8–1.1. The OD₆₀₀ was followed over 95 h. N = 2 independent experiments