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. 2020 Dec 21;13:208. doi: 10.1186/s13068-020-01851-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Western Blot analysis of Rnf subunits and detection of FMN in Rnf subunits of the purified Rnf complex from A. woodii. Purified proteins (20 µg) were separated on an SDS-PAGE and were either transferred onto nitrocellulose membranes and blotted against His₆, RnfC, RnfB, and RnfG antibodies (a) or were placed under UV light (280 nm) for visualization of flavin-containing subunits (b)