Table 2.

Univariate analyses of metabolites associated with sheep RFI

	Metabolite	Fold change	P-value	Metabolite Category	Platform
1	Alpha-aminoadipic acid	1.88	<0.001	Biogenic amines	DI/LC-MS/MS
2	Ketoleucine	1.72	<0.05	Ketones	NMR
3	Acetone	1.78	<0.005	Ketones	NMR
4	Isopropyl alcohol	20.83	<0.001	Alcohol or polyol	NMR
5	Cesium (Cs)	<1.5	<0.001	Metal	ICP-MS
6	C5 (Valerylcarnitine)	<1.5	<0.001	Acylcarnitines	DI/LC-MS/MS
7	C5-OH (C3-DC-M) (Hydroxyvalerylcarnitine)	<1.5	<0.001	Acylcarnitines	DI/LC-MS/MS
8	PC aa C40:2	<1.5	<0.001	Phospholipids	DI/LC-MS/MS
9	C6 (C4:1-DC) (Hexanoylcarnitine)	<1.5	<0.001	Acylcarnitines	DI/LC-MS/MS
10	C0 (Carnitine)	<1.5	<0.001	Acylcarnitines	DI/LC-MS/MS
11	Asymmetric dimethylarginine (ADMA)	<1.5	<0.001	Biogenic amines	DI/LC-MS/MS
12	Spermidine	<1.5	<0.005	Biogenic amines	DI/LC-MS/MS
13	PC ae C36:0	<1.5	<0.005	Phospholipids	DI/LC-MS/MS
14	PC aa C40:1	<1.5	<0.005	Phospholipids	DI/LC-MS/MS
15	C14:1-OH (Hydroxytetradecenoyl carnitine)	<1.5	<0.005	Acylcarnitines	DI/LC-MS/MS
16	Glycerol	<1.5	<0.005	Sugar alcohols	NMR
17	C16 (Hexadecanoylcarnitine)	<1.5	<0.005	Acylcarnitines	DI/LC-MS/MS
18	LysoPC a C16:1	<1.5	<0.005	Phospholipid	DI/LC-MS/MS
19	PC aa C32:2	<1.5	<0.005	Phospholipid	DI/LC-MS/MS
20	Copper (Cu)	<1.5	<0.005	Metal	ICP-MS
21	Potassium (K)	<1.5	<0.005	Metal	ICP-MS
22	LysoPC a C18:1	<1.5	<0.005	Phospholipid	DI/LC-MS/MS
23	PC ae C40:6	<1.5	<0.005	Phospholipid	DI/LC-MS/MS
24	Hippuric acid	<1.5	<0.005	Benzenoid	NMR
25	Taurine	<1.5	<0.005	Biogenic amine	DI/LC-MS/MS

These metabolites differentiated between high-RFI and low-RFI sheep.