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. 2020 Jul 17;12(8):630–643. doi: 10.1093/jmcb/mjaa036

Figure 2.

Figure 2

Culturing of human colon organoids in a 384-well plate format. (A) Scheme for miniaturizing organoid culture in a 384-well plate. Organoids growing in BME gel in the 24-well plate were collected and re-suspended in ice-cold BME gel. The organoid cells/BME gel mixture (8 µl/well) was dispensed into a 384-well plate. After incubation for 30 min at 37°C, warm organoid culture medium (35 µl) was dispensed to each well. The plate was incubated for up to 8 days without replacing the medium. (B) The organoids growing in a 384-well plate was monitored using ImageXpressmicro and showed progressing growth over the time. (C) The growth of organoids in 384-well plate was quantified by CellTiter Blue cell viability assay and showed robust FI signals for HTS with Zʹ of 0.7 and S/B ratio of 6.3.