Figure 3.

Screening of the EEBL library with the human colon organoids in a 384-well plate format. Human colon APC^−/−;KRAS^G12D organoids cultured in 384-well plates were treated with library compounds (0.1 µl, final at 4.6 µM). The plates were incubated for 3 days and subjected to CellTiter Blue cell viability assay. The effect of compounds on the growth of KRAS^G12D organoids was calculated as % Inhibition based on each plate and analyzed using Cambridge BioAssay software. (A and B) Performance evaluation with Zʹ factors (A) and S/B (B) across six 384-well screening plates. (C) Scatter plot of compound effects (% Inhibition) from the HTS. (D) An example plate hit map. (E) Correlation between two independent screens.