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. 2020 Jul 17;12(8):630–643. doi: 10.1093/jmcb/mjaa036

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© The Author(s) (2020). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Imaging analysis of cell fate of organoids in a 384-well plate format. Organoids growing in a 384-well plate (4 days) were treated with DMSO, MLN9708, or ABT-263 (5 µM) for 3 days. A combination of dyes diluted in PBS containing Hoechst 33342, Calcein-AM, and PI was added and incubated for 30 min. The bright field and fluorescence images were captured with the ImageXpress^micro automated imaging system using Z-stack. The representative merged images from Z-stack for DMSO control, MLN9708, and ABT-263 treatment are shown.