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. 2020 Jul 17;12(8):630–643. doi: 10.1093/jmcb/mjaa036

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© The Author(s) (2020). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Miniaturized 3D organoid assays in a 1536-well plate format for uHTS. (A) Growth of human colon APC^−/−;KRAS^G12D organoids in 1536-well vs. 384-well plates was compared. (B) The effect of compounds, ABT-263 as an example, exhibited very similar inhibitory effect on the growth of APC^−/−;KRAS^G12D organoids between 384-well and 1536-well plate formats. (C) Performance evaluation for the uHTS viability assay with organoids showing Zʹ (>0.5) and S/B (>5) scores across the screening plates. (D) Scatter plot of primary screening of a 10240-diversity compound library in a 1536-well uHTS format. Screening data were analyzed using Cambridge BioAssay software. The effect of compounds on the growth of APC^−/−;KRAS^G12D organoids was calculated as % Inhibition per plate.