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. 2020 Dec 10;28(5):509–518. doi: 10.3727/096504020X15954139263808

Figure 4.

Figure 4

miR-186 directly targets CDK6. (A) The putative conserved miR-186-binding site in the CDK6 3′-untranslated region (3′-UTR). (B) Western blotting was used to analyze the relative expression of CDK6 in HepG2 and HUH7 cells transfected with miR-186 or NC mimics, respectively. (C) The relative luciferase activity of 293T cells cotransfected with miR-186 mimics/NC and CDK6 wild type (WT)/mutant (MUT) was detected using luciferase reporter assay. Data represent means ± SEM of three independent experiments. *p < 0.01.