Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| phospho-S6 | CST | Cell Signaling Technology Cat# 2215L, RRID:AB_331683 |
| total S6 | CST | Cell Signaling Technology Cat# 2217, RRID:AB_331355 |
| phospho-ERK | CST | Cell Signaling Technology Cat# 4370 |
| total ERK | Santa Cruz | Santa Cruz Biotechnology Cat# sc-94, RRID:AB_2140110 |
| GCK | Santa Cruz | Santa Cruz Biotechnology Cat# sc-7908, RRID:AB_2107620 |
| SREBP-1 | BD Pharmingen | clone IgG-2A4 |
| Actin | Abcam | Abcam Cat# ab1801, RRID:AB_302617 |
| Chemicals, Peptides, Recombinant Proteins | ||
| Insulin (Humulin R) | Lilly | NDC 0002-8215-01 |
| D-Glucose | Sigma | G8270 |
| AMPure XP beads | Beckman Coulter | A63881 |
| TruSeq Stranded mRNA Library Prep | Illumina | 20020595 |
| SuperScript II | Invitrogen | 18064022 |
| Critical Commercial Assays | ||
| Glucose, serum | ThermoFisher Scientific, Infinity Glucose Hexokinase |
TR15421 |
| Cholesterol, serum | ThermoFisher Scientific, Infinity Cholesterol |
TR13421 |
| Triglycerides, serum | ThermoFisher Scientific, Infinity Triglycerides |
TR22421 |
| Triglycerides, liver | Stanbio™ Triglycerides LiquiColor™ Test (Mono) Reagents |
SB2200225 |
| Insulin, Leptin | MSD, Mouse metabolic kit | K15124C |
| Adiponectin | MSD, Mouse adiponectin kit | K152BXC |
| Deposited Data | ||
| Liver RNA seq | This study | GEO: GSE102072 |
| Experimental Models: Organisms/Strains | ||
| Cry1;Cry2 double KO (CDKO) | Gift from Dr. Sancar, Vitaterna et al., 1999 |
|
| Rev-erbα/β double floxed mice | Gift from Dr. Evans, Cho et al., 2012 | |
| Bmal1l°x | Storch et al., 2007 | Jax strain #007668; RRID:IMSR_JAX:007668 |
| Albumin-Cre | Postic et al., 1999 | Jax strain #003574; RRID:IMSR_JAX:003574 |
| Oligonucleotides | ||
| Genotyping primers | ||
| Rev-erbα - Fw: CATTACCAGTAAGTCAATGCCCAGC |
Cho at al., 2012 | |
| Rev-erbα - Rev: GAAGAGTGTGTGTTTGCCCAAGAGG |
Cho at al., 2012 | |
| Rev-erbβ - Fw: TCATCGCTCCAGTCTCCTACATTTC |
Cho at al., 2012 | |
| Rev-erbβ - Rev: GGAAGTGCTCCAACAAGGTAGTGCA |
Cho at al., 2012 | |
| Cry1_WT_Fwd1: GATTCACTGTGTGTTGAGCAAGG |
||
| Cry1_exonb_Rev1: CTTGAGTGAGTGAGTCTGCTG |
||
| Cry1_Neo_Fwd: CCTTCTATCGCCTTCTTGACGAG |
||
| Cry2_Rev: CACACTGAAATCGGCATCCAG |
||
| Cry2_Fwd1: GCAAGACAGGCTTCCCTTGG |
||
| Bmal1 - Fw: ACTGGAAGTAACTTTATCAAACTG |
Storch et al., 2007 | JAX oIMR7525 |
| Bmal1 - Rev: CTGACCAACTTGCTAACAATTA |
Storch et al., 2007 | JAX oIMR7526 |
| Cre - Fw: GAACCTGATGGACATGTTCAG |
||
| Cre - Rev: AGTGCGTTCGAACGCTAGAGC |
||
| Software and Algorithms | ||
| Fiji (ImageJ) | NIH | RRID: SCR_002285, http://fiji.sc/ |
| Prism | GraphPad | RRID: SCR_015807, https://www.graphpad.com/scientific-software/prism/ |
| R | R Foundation for Statistical Computing | RRID: SCR_001905, https://www.r-project.org |
| FastQC | Babraham Bioinformatics | RRID: SCR_014583, http://www.bioinformatics.babraham.ac.uk/projects/fastqc |
| STAR aligner | Dobin et al., 2013 | RRID: SCR_015899, https://github.com/alexdobin/STAR |
| HOMER | Heinz et al., 2010 | RRID: SCR_010881, http://homer.ucsd.edu/homer/ |
| MetaboAnalyst | Xia et al., 2016 | RRID: SCR_015539, http://www.metaboanalyst.ca |
| Metascape | Tripathi et al., 2015 | http://metascape.org |
| Other | ||
| normal chow diet | Lab Diet | 5053 |
| 60% high fat diet | TestDiet | 58Y1 |