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. Author manuscript; available in PMC: 2020 Dec 21.
Published in final edited form as: Circ Res. 2014 Dec 17;116(5):827–835. doi: 10.1161/CIRCRESAHA.116.305825

Figure 2. Plasma annexin A1 (AnxA1) negatively correlates with atherosclerotic lesion sizes.

Figure 2.

A, Apoe−/− and Apoe−/−Anxa1−/− mice were fed a high-fat diet for 4 weeks, and aortic root sections were stained with an antibody to AnxA1 or an isotype control antibody. B, Apoe−/− mice were fed a high-fat diet (HFD) for 4 weeks and AnxA1 was costained with markers for macrophages (anti-Mac2), smooth muscle cells (anti-αSMA), endothelial cells (anti-von Willebrand Factor [vWF]), or neutrophils (anti-Ly6G) in aortic root sections. Scale bar, 100 μm. C and D, Apoe−/− and Apoe−/−Fpr2−/− mice were fed a HFD for 4 weeks and AnxA1 was quantified in the plasma (B). Correlation between aortic root lesion sizes and plasma AnxA1 levels in Apoe−/− and Apoe−/−Fpr2−/− mice (Pearson correlation; D). DAPI indicates 4’,6-diamidino-2-phenylindole.