Table 3.
Effects of various inhibitors and detergents on the activity of lipase.
| Relative activity (%) |
|||
|---|---|---|---|
| 1 mM (0.1%, v/v) | 10 mM (1%, v/v) | ||
| Control | 100.0 ± 1.5 | 100.0 ± 1.0 | |
| Inhibitors | |||
| DTT | 85.3 ± 2.1 | 71.5 ± 1.7 | |
| EDTA | 66.5 ± 2.4 | 32.9 ± 3.3 | |
| H2O2 | 88.9 ± 1.1 | 76.8 ± 1.4 | |
| Detergents | |||
| CTAB | 107.8 ± 2.0 | 91.5 ± 0.6 | |
| SDS | 94.6 ± 0.2 | 89.2 ± 3.1 | |
| Sodium cholate | 86.6 ± 2.6 | 80.3 ± 4.4 | |
| Tween-20 | 104.3 ± 1.3 | 94.5 ± 1.0 | |
| Tween-60 | 97.6 ± 2.2 | 92.1 ± 1.9 | |
| Tween-80 | 120.3 ± 0.8 | 105.7 ± 3.4 | |
| Triton X-100 | 104.9 ± 1.7 | 96.2 ± 1.9 | |
Inhibitors and detergents (1 and 10 mM, 0.1 and 1% v/v) were added in the reaction mixture and assayed using standard assay protocol. Results are presented as means ± standard deviation (n = 3).